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• QuantiScan是一款操作简单、功能专一的凝胶扫描分析软件。QuantiScan还可以帮你分析，扫描多种多样的材料成分。可以将普通的办公扫描仪转换为功能强大的显像密度计，用于分析凝胶，污点和薄层层析板等。
• 用于Windows的QuantiScan具有复杂的光密度计相似的功能，它能够分析多种材料，包括聚丙烯酰胺和琼脂糖凝胶，放射自显影图，TLC板等。该程序将直接从任何TWAIN兼容扫描仪以及大多数BMP，JPG或未压缩的TIF文件接受图像进行分析。对于质量合理的泳道凝胶，分析是高度自动化的，可实现极高的数据通量。使用Quantiscan的手动模式几何可以分析任何类型的图像。
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• 数字，图形和图像输出可以打印为报告，也可以另存为文件或复制到剪贴板。可以将光密度分析分布图的X，Y坐标输出到选项卡式表格中，以便在演示图形软件（例如Biosoft的FigSys）中进行重新绘图。有一个单独的模块，用于分析不连续的印迹，也可以通过自动或手动印迹检测进行操作。
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• QuantiScan for Windows has similar functionality to sophisticated densitometers but at a fraction of the cost. It is able to analyze a wide variety of material including polyacrylamide and agarose gels, autoradiograms, TLC plates, etc. The program will accept images for analysis directly from any TWAIN compliant scanner as well as most BMP, JPG or uncompressed TIF files. For laned gels of reasonable quality, the analysis is highly automated, allowing extremely high throughput of data. Almost any type of image can be analyzed with the manual modes of Quantiscan. Images can be pre-processed in a variety of ways within QuantiScan including crop, flip, invert, scroll and zoom.
• Numeric, graphical and image output can be printed as reports and can also be saved as files or copied to the clipboard. The X,Y coordinates of densitometric profiles can output into a tabbed table for re-plotting in presentation graphics software such as Biosoft's FigSys. There is a separate module for analysis of discrete blots which can also be operated with automatic or manual blot detection.
• Lane Analysis Mode
• There is sophisticated lane processing and analysis for gels. Lanes of any width can be created manually or by using the unique automatic lane detection mode. There is also a facility to automatically set lanes at regular intervals for use with plates that have a preconfigured regular lane array. Lanes can be skewed or curved to allow for gel imperfections. Lane boundaries can be copied and the copies moved precisely into position with the cursor keys, for exact comparisons. Each lane can be labelled with an unique number both on its image and its densitometric profile plot. There are six options for automatic background subtraction: Minimum, Interpolated minimum, Valley, Edge, Polynomial and Manual. User-selected areas of images can be displayed as 3-D density maps which can be printed, saved or copied to the clipboard.
• Once a lane has been defined, its densitometric profile plot is automatically drawn. Peaks can then be specified on the profile either manually by marking their center and edges, or automatically using a sophisticated peak search routine. Where appropriate, automatic fitting of Gaussian peaks can be performed. This automatic fitting uses a least-squares minimization technique to fit a sequence of peaks and a polynomial background to the given profile. The image of the currently-selected lane is shown laid-out horizontally under the lane densitometry profile. A cursor-controlled vertical line going across the profile and the lane image aids user examination of the peaks. The image and profile of a standard lane can be added for comparison with the first. Their alignment can be adjusted to aid accurate side-by-side comparisons. Peak areas are calculated by integration using Simpson's rule and, if the automatic fitting option has been used, the area of the fitted peak is also automatically calculated.
• Calibration to a lane containing standard proteins may be made for automatic calculation of molecular weights. Calibration can also be made with a known standard solution such as those supplied with assay kits (just enter the position of its start line, its mol. wt. factor and the position of the solvent front, to give automatic calculation of mol. wts of unknowns). Parameters of any standard lane can be saved for between-plate comparisons. Calibration curve graphs and their fitted coordinates can be exported. Reports are generated for all peak parameters, including position, height, width, background and net area of peaks and, together with the graphical results, these can be printed, saved to disk or copied to the clipboard for transfer to other programs.
• Blot Analysis Mode
• QuantiScan offers a separate mode to analyze discrete blots. The blots can be automatically detected or manually defined. Their number, position, height, area and densitometric volume are automatically reported. There is a special module for automatic analysis of regularly arrayed blots. Automatic background subtraction is an option. Calibration, based on densitometric volume of standard blots can be done and this allows blot mode to be used for assays.